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Interphase Interphase is the phase of the cell cycle in which the cell spends and performs the majority of its time. Then, in preparation for cellular division, it increases in size. Most of the cell stay in the interphase. The interphase is in the chromosomes copied and the cell get larger. Originally this phase of the cell cycle was called the "resting stage", since light microscopy could not detect any activities taking place within the cells. Today, however, it is known as a stage of considerable activity at the molecular and sub- cellular level and is usually subdivided into: G 1 , S, and G 2 . G 1 is a period of molecular synthesis where a newly formed cell turns on a variety of genes on its DNA to make proteins, which in turn churn the metabolism of the cell, produce and breakdown carbohydrates, lipids, etc., and transform energy from food into ATP. The cell grows and enlarges. The G 1 is gap one. S is during this phase the chromatin (DNA and proteins) becomes synthetically active. Using elaborate teams of enzymes, the DNA molecules of each chromosome are copied by semiconservative DNA synthesis. This phase cannot be clearly seen or distinguished under the light microscope, even with DNA stains, as the material is too diffuse. However, the making of new DNA molecules can be monitored by following the incorporation of radioactive isotopes into the newly forming DNA molecules. S is synthesis. G 2, gap two, is another period, of variable length, in which cells prepare for division. Many different proteins are synthesis, especially those that will act as spindle fibers (protein "ropes
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